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KMID : 0613820070170050664
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Journal of Life Science
2007 Volume.17 No. 5 p.664 ~ p.670
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Radioprotective Effects of Propolis on the Mouse Testis Exposed to X-ray
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Ji Tae-Jeong
Kim Jong-Sik
Jeong Hyung-Jin
Seo Eul-Won
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Abstract
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The propolis is natural product produced by honeybees and is known to have many biologically useful properties such as anti-microbial, anti-oxidative and anti-tumorigenic activity. However, its radio-protective property has not been well studied. To investigate radio-protective effect of propolis on mouse testis, mice were supplemented with propolis after 5 Gy irradiation. The histological changes of testis were detected by TEM. The results indicate that propolis may protect tissue deformation which is induced by 5 Gy of ionizing radiation. Furthermore, to elucidate the potential molecular mechanisms involved in radio-protective property of propolis, we performed microarray experiments using oligo DNA microarray. We found 65 up-regulated genes and 224 down-regulated genes, whose expression levels were affected more than 2-fold by propolis treatment in mice irradiated at 5 Gy. We confirmed microarray data with reverse transcription-PCR using gene specific primers. The results of RT-PCR are highly correlated with those of microarray. These results may help understanding molecular mechanisms of radioprotective effects by propolis in mouse model.
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KEYWORD
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propolis, radio-protection, microarray, testis
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